Peptide mapping using Liquid Chromatography-Mass Spectrometry (LC-MS) is an essential analytical technique for confirming the amino acid sequence of proteins and characterizing their post-translational modifications (PTMs). This method combines the high separation power of liquid chromatography with the sensitive mass spectrometric detection of peptides, allowing for the detailed analysis of protein structure and modifications.
Key Benefits of Peptide Mapping LC-MS:
- Amino Acid Sequence Verification: Ensures the correct protein sequence, which is crucial for the consistency and safety of therapeutic proteins, such as monoclonal antibodies.
- Comprehensive PTM Profiling: Detects and characterizes PTMs, which are essential for understanding protein function, stability, and activity.
- Detailed Structural Information: Provides both primary sequence and higher-order structural insights, essential for understanding the function and therapeutic potential of proteins.
- Quality Control in Biopharmaceuticals: Helps ensure batch-to-batch consistency in therapeutic proteins by detecting any sequence variations or modification inconsistencies that could affect product quality.
In peptide mapping, enzymatic digestion is a crucial step to break down proteins into smaller peptides that can be analyzed by LC-MS.
Aragen offers a comprehensive solution to identify and fragment target peptides and their modifications by selecting highly specific proteases. This approach ensures precise confirmation of both the primary structure and post-translational modifications, delivering a high degree of accuracy and confidence.
The most used enzymes in peptide mapping are Trypsin, Chymotrypsin, Lys-C, Asp-N, Glu-C, Arg-C etc.
The mass estimation of target peptide can be accurately estimated with less than 20ppm mass error to in-line with the regulatory expectations.
