Surface Plasmon Resonance (SPR) is a powerful and widely used technique for studying molecular interactions, particularly to measure the binding affinity and kinetics between biological molecules, such as proteins, nucleic acids, and small molecules. Here is a brief overview of how it works:
SPR measures changes in the refractive index near the sensor surface, where one binding partner (the ligand) is immobilized, and the other (the analyte) is injected in solution. When the analyte binds to the immobilized ligand, the mass change at the surface leads to a shift in the resonance angle of the reflected light, which is detected in real-time.
Advantages:
- Real-Time Data: SPR allows for real-time monitoring without the need for labels.
- High Sensitivity: It can detect interactions at low concentrations of analyte (nanomolar to picomolar).
- No Labelling Required: SPR measures binding directly, avoiding potential artifacts from labelling.
- Reproducible: SPR data shows highly reproducible with accurate measurements which can be useful for regulatory submissions.
Fc binding affinity, which refers to the interaction between the Fc region of an antibody and its target, such as Fc receptors (FcRs) or other proteins that bind to the Fc domain. The Fc region is responsible for mediating the immune effector functions of antibodies.
FcRn (Neonatal Fc Receptor) is an important receptor that plays a key role in the half-life extension and recycling of IgG antibodies in the bloodstream. It is primarily involved in protecting antibodies from degradation and in transporting them across epithelial barriers.
C1q binding affinity study is part of the classical pathway of complement activation. It binds to the Fc region of IgG antibodies. Upon binding to the Fc region of antibodies, C1q activates the C1 complex, triggering the cascade of complement proteins and leading to immune responses such as opsonization, pathogen clearance, and inflammation.
Aragen can provide the seamless support on binding affinity studies by SPR of your molecules, our team is fully expertise in using the various chemistry like; Amine Coupling (Covalent Coupling) & Biotin-Streptavidin (avidin) and we follow appropriate orientation of bindings like ligand-analyte or analyte-ligand with single cycle kinetics/ multi cycle kinetics approach