Clostridioides difficile Infection (CDI) Model
Clostridioides difficile is a gram-positive, spore-forming bacterium that causes infections primarily associated with antibiotic use, leading to symptoms ranging from mild diarrhea to severe colitis. To study C. difficile infection (CDI), animal models, particularly mice and hamsters, are widely used due to their ability to mimic the human disease, allowing researchers to investigate pathogenesis, host responses, and potential treatments. The hamster model is a traditional model used in C. difficile research due to its high susceptibility to the bacterium, making it ideal for studying severe disease outcomes. The mouse model allows for genetic manipulation, such as knockout or transgenic mice, to study host-pathogen interactions and immune responses. It is also suitable for testing new therapies and vaccines.
By partnering with Aragen, we help you enhance your research capabilities in CDI, ensuring reliable results that pave the way for innovative therapies. Our expert team is dedicated to supporting your research needs, providing you with the tools to advance your understanding of C. difficile and improve patient outcomes.
Methodology
- D -1: Pretreatment with Clindamycin
- D 0: Spore administration (oral gavage)
- Drug administration (i.p., oral gavage, etc.)
- Monitoring of animals:
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- For daily food intake and change in body weights
- Twice daily observation for onset of diarrhea and general activity levels
- Collected fecal pellets
- Optional: Harvest of organs for histopathology
- Evaluation of additional parameters (e.g., Toxin-detection)
Results
- Vehicle treatment resulted in 0% survival and Vancomycin treatment resulted in 100% survival as expected.
- Test articles (TA 1-5) resulted in varying levels of protection from CDI infection.
Methodology
- Day -7 to Day -2: pre-treatment Cefoperazone, drinking water.
- Day 0: Spore administration (oral gavage) VPI 10463
- Day 2-6 Vancomycin, drinking water
Results
- Vehicle treatment resulted in body weight loss and 30% survival.
- Vancomycin treatment reduced body weight loss and results in 90% survival
Citrobacter Infection-induced Colitis Model in Mice
The Citrobacter rodentium infection model is a widely used experimental system to study gastrointestinal diseases and host-pathogen interactions. This model mimics several aspects of human intestinal disorders, making it a valuable tool for researchers. Key applications of the C. rodentium model include:
- Investigating Host–Pathogen Immune Interactions in the Gut: C. rodentium infection closely resembles human enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) infections, allowing the study of host immune responses, including innate and adaptive immunity. This model helps unravel the mechanisms by which the immune system recognizes and responds to gut pathogens, providing insights into the balance between immune defense and inflammation.
- Understanding the Pathogenesis of Human Intestinal Disorders such as inflammatory bowel diseases (IBD) such as Crohn’s disease and ulcerative colitis:
Overall, the C. rodentium model is a powerful tool for studying gut immune interactions and the pathogenesis of human intestinal diseases, enhancing our understanding of host-pathogen dynamics and the development of targeted therapies.
At Aragen, we enhance your research capabilities with our advanced C. rodentium infection model in mice. By choosing our services, gain access to a meticulously designed study that supports the development of targeted therapies against enteric pathogens. Collaborate with us to drive innovation and make meaningful contributions to gastrointestinal health.
Methodology
- Day 0: Oral administration of C. rodentium in immunocompetent mice
- Monitor weight and survival throughout the study
- Test articles administered PO, SC, IV etc.
- Day of harvest options: Colon weight/length ratio, intestinal permeability, histopathology, immune cell analysis, cytokine analysis, bacterial load.
Result
- Infection with C. rodentium resulted in body weight loss and increased colon weight/length ratio.